Journal: Gene therapy

Article Title: Tumor cells expressing a fusion protein of MULT1 and Fas are rejected in vivo by apoptosis and NK cell activation.

doi: 10.1038/gt.2008.77

Figure Lengend Snippet: Figure 3 Mouse UL16-binding protein-like transcript 1 E (MULT1E)/FasTI induces apoptosis. A total of 1 106 cells of TC-1 and clones L-5, L-7 and L-10 were treated with 1 mg/ml NKG2D/Fc for 16 h. The cells were then analyzed for apoptosis and necrosis using Annexin V assay (a–c) or caspase-3 assay (d) according to the manufacturers’ protocols. (a) An example of the fluorescence-activated cell sorting (FACS) data. (b, c) Summaries of data from three separate experiments. The statistical analyses were conducted between the controls (open bars) and NKG2D/Fc-treated cells (solid bars) using two-way analysis of variance (ANOVA). The difference between NKG2D/Fc-treated L-5 cells and NKG2D/Fc-treated L-10 cells was also compared using Student’s t-test. *Po0.05; **Po0.01 and ***Po0.001.

Article Snippet: Induction of apoptosis in cells expressing the fusion protein To determine if cells expressing the fusion protein can be induced to undergo apoptosis, one million cells of TC-1 and clones L-5, L-7 and L-10 were treated with 1 mg/ml of NKG2D/Fc for 16 h. Apoptosis of the cells was measured using two systems: a TACS Annexin V-FITC Apoptosis Kit (R&D Systems) and a caspase-3 fluorometric assay (R&D Systems).

Techniques: Binding Assay, Clone Assay, Annexin V Assay, Caspase-3 Assay, FACS

Journal: Scientific Reports

Article Title: Ablation of TRPC3 disrupts Ca 2+ signaling in salivary ductal cells and promotes sialolithiasis

doi: 10.1038/s41598-023-32602-8

Figure Lengend Snippet: Calcification due to TRPC3 ablation upregulates oxidative stress and apoptosis in SMG. Densitometric analysis of ( A–C ) RT-PCR and/or ( D ) protein blot expressions for oxidative stress markers (CHOP, NOX4, FMO2, GPX3, GPX6, m18S, and SCD1) or apoptotic markers (BAX1/BCL2, Cas3, and Cas12) in WT and TRPC3 -/- (KO) mice SMG tissues were performed using ImageJ. GAPDH and β-actin were utilized as internal control for RT-PCR and immunoblotting, respectively. Each RT-PCR and immunoblotting experiment was performed from n = 3 mice. Full length blots are indicated in the Supplementary Materials. ( A–D ) Bar diagrams of quantitated RT-PCR or western blots depicted in mean + SEM. * P < 0.05; ** P < 0.01. ( E ) in situ apoptosis was detected in mice WT and KO SMG tissues.

Article Snippet: In situ apoptosis in mice tissue sections were performed using TACS•XL DAB in situ Apoptosis Detection Kit (Trevigen, Gaithersburg, MD, USA), as per manufacturer’s directions and our previously published method on mice kidney tissue section .

Techniques: Reverse Transcription Polymerase Chain Reaction, Control, Western Blot, In Situ